See Change® in Protein Characterization with the Microfluidic Modulation Spectroscopy-based AQS3TMpro
When it comes to measuring protein secondary structure, there is an abundance of tools available in today’s laboratories, most commonly spectroscopy, in the form of UV Vis, Circular Dichroism (CD), or FTIR spectrophotometers. Techniques such as Raman spectroscopy are becoming more accepted with recent improvements in lasers and detectors. Even NMR has taken a step into large molecule structural analysis, with multi-dimensional NMR techniques and improved data processing.
Many of these techniques, however, come with their own limitations. CD, for instance, has a limited concentration range of about 0.1 to 0.5 mg/mL. Commonly found excipients in biopharmaceuticals such as surfactants, sugars, and amino acids are often optically active, limiting the measurement of CD on formulations. To measure a protein in its formulations conditions, Fourier-transform Infrared Spectroscopy (FTIR) may provide a solution, but not without its own challenges. FTIR is commonly a ected by background drift, making extended measurements over time challenging. Additionally, the concentration range measurable using FTIR restricts samples to greater than 10mg/mL. This limits its application to early discovery and development phases of biopharmaceutical manufacturing where such volumes are not available.
Microfluidic Modulation Spectroscopy as seen in the AQS3TMpro system from RedShiftBio is a novel and efficient technique for label- free protein analysis that directly addresses the limitations of current technologies. MMS provides drift-free, background subtracted, high sensitivity measurements of the secondary structure of proteins across four decades of concentration from 0.1 to >200 mg/mL.
- Automated multi-sample analysis for walk-away operation
- Increased sensitivity to see change®
- Widest concentration range to characterize biotherapeutics
- Analytical software that easily transitions data into insight
The AQS3TMpro will change how you measure protein and peptide structure. Powered by MMS, it ramps up the sensitivity, dynamic range, accuracy, and utility of IR spectroscopy. Designed for five key measurements—aggregation, quantitation, stability, similarity, structure—the AQS3TMpro directly supports protein research and progress through the biopharmaceutical development pipeline.
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